Tissue and subcellular distribution of the lectin from Datura stramonium (thorn apple)
نویسندگان
چکیده
منابع مشابه
Purification of the lectin from Datura stramonium.
The lectin from Datura stramonium can be inhibited by oligomers of N-acetylglucosamine. This property was exploited to purify the lectin by affinity chromatography on Sepharosefetuin. The purified lectin is a glycoprotein in having subunits of 40 000 and 45 000 mol.wt.
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1. Methylation analysis of potato (Solanum tuberosum) lectin and thorn-apple (Datura stramonium) lectin confirmed previous conclusions that both glycoproteins contained high proportions of l-arabinofuranosides and lesser amounts of d-galactopyranosides. The arabinofuranosides are present in both lectins as short unbranched chains containing 1-->2- and 1-->3-linkages, which are known to be linke...
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The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The dimeric lectin (Mr = 86,000) possesses two carbohydrate-binding sites for N,N'N",N"'- tetraacetylchitotetritol /mol protein, with an apparent Ka = 8.7 X 10(3) M-1 at 4 degrees...
متن کاملPurification and some properties of a lectin from the fruit juice of the tomato (Lycopersicon esculentum).
In the tomato (Lycopersicon esculentum) plant, the fruit juice was found to be the richest source of agglutinating activity. The lectin responsible could be inhibited by oligomers of N-acetylglucosamine, and this property was exploited to purify the lectin by affinity adsorption on trypsin-treated erythrocytes. The lectin is a glycoprotein that cross-reacts immunologically with the lectin from ...
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partaken of a quantity of datura leaves, prepared in the form of curry, with rice, which he afterwards acknowledged to have voluntarily taken. On admission, he lay perfectly unconscious, with severe spasmodic muscular twitchings and stertorous breathing. Skin cool; pulse about 108?, feeble. Pupils dilated and insensible to the effects of light. The stomach pump was immediately introduced, but d...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0306-3283
DOI: 10.1042/bj1840215